Journal: Frontiers in Neuroscience

Article Title: Reduced acetylated α-tubulin in SPAST hereditary spastic paraplegia patient PBMCs

doi: 10.3389/fnins.2023.1073516

Figure Lengend Snippet: Mouse noscapine treatment study plan.

Article Snippet: The PBMCs were treated with 10 μM noscapine (Cayman chemical, Catalog# 17255) for 1 h.

Techniques:

Journal: Frontiers in Neuroscience

Article Title: Reduced acetylated α-tubulin in SPAST hereditary spastic paraplegia patient PBMCs

doi: 10.3389/fnins.2023.1073516

Figure Lengend Snippet: Plasma and brain noscapine levels in noscapine treated mouse. Mouse was orally administered with increasing concentrations of noscapine: 100, 200, and 400 mg/kg. Noscapine was measured in mouse plasma (A) and brain homogenate (B) at 60 min post-dose using Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS). N = 4 mouse per group. One way ANOVA confirmed a significant noscapine treatment effect in the mouse plasma ( p < 0.01) and brain ( p < 0.01). Tukey’s post-hoc multiple comparisons showed group differences ( * p < 0.05 and ** p < 0.01). Mean ± SEM.

Article Snippet: The PBMCs were treated with 10 μM noscapine (Cayman chemical, Catalog# 17255) for 1 h.

Techniques: Clinical Proteomics, Liquid Chromatography, Mass Spectrometry, Liquid Chromatography with Mass Spectroscopy

Journal: Frontiers in Neuroscience

Article Title: Reduced acetylated α-tubulin in SPAST hereditary spastic paraplegia patient PBMCs

doi: 10.3389/fnins.2023.1073516

Figure Lengend Snippet: Correlation of noscapine levels in plasma and brain of noscapine treated mouse.

Article Snippet: The PBMCs were treated with 10 μM noscapine (Cayman chemical, Catalog# 17255) for 1 h.

Techniques: Clinical Proteomics

Journal: Frontiers in Neuroscience

Article Title: Reduced acetylated α-tubulin in SPAST hereditary spastic paraplegia patient PBMCs

doi: 10.3389/fnins.2023.1073516

Figure Lengend Snippet: Acetylated α-tubulin levels in noscapine treated mouse. Acetylated α-tubulin was measured in the brain of the mouse dosed with noscapine. Mouse was orally administered with increasing concentrations of noscapine: 100, 200, and 400 mg/kg. One way ANOVA confirmed a significant noscapine treatment effect on acetylated α-tubulin in the mouse brain ( p < 0.01). Tukey’s post-hoc multiple comparisons showed group differences ( * p < 0.05, ** p < 0.01, *** p < 0.001). N = 3 or 4 mouse per group. a.u. = arbitrary unit. Mean ± SEM.

Article Snippet: The PBMCs were treated with 10 μM noscapine (Cayman chemical, Catalog# 17255) for 1 h.

Techniques:

Journal: Frontiers in Neuroscience

Article Title: Reduced acetylated α-tubulin in SPAST hereditary spastic paraplegia patient PBMCs

doi: 10.3389/fnins.2023.1073516

Figure Lengend Snippet: Acetylated α-tubulin levels in noscapine treated human PBMCs. Acetylated α-tubulin was measured in human PBMCs treated with noscapine ( in vitro ) at 10 μM for 1 h. Student’s t-test confirmed a significant noscapine treatment effect on acetylated α-tubulin levels ( **** p < 0.0001). N = 8 PBMC samples per untreated and noscapine treated groups. Mean ± SEM.

Article Snippet: The PBMCs were treated with 10 μM noscapine (Cayman chemical, Catalog# 17255) for 1 h.

Techniques: In Vitro

Journal: WormBook : the online review of C. elegans biology

Article Title: Transgenic solutions for the germline *

doi: 10.1895/wormbook.1.148.1

Figure Lengend Snippet: Selected Entry Clones

Article Snippet: spe-11 promoter , in sperm , 272 bp spe-11 promoter. , pCM1.41 ( ) , Addgene ( http://www.addgene.org/pgvec1 ).

Techniques: Clone Assay, Expressing, Sequencing